Metrohm^® Biotrode Electrodes

Biotrode—outstanding features in a small package

The Metrohm Biotrode (6.0224.100) combination glass electrode brings highly accurate and quick measurements of a larger electrode system to the realm of small biological samples.

Applications

• Measurements in very small sample volumes
• Biology/Protein pH measurements
• Biotechnology
• Pharmaceutical

Small diameter
Designed with a 3 mm diameter immersion system, the Biotrode is well suited for pH measurements in very small volumes down to 60 µl. It can be easily used in sample vessels with small diameters, such as microplates, for quick analysis.

Quick and accurate
The Biotrode uses a spherical glass membrane manufactured from a special low-impedance membrane glass (M Glass) to ensure short response times. This new electrode also uses the Metrohm “Long Life” reference system with the Ag/AgCl cartridge; its silver ion barrier ensures an extended life and consistent measurements.

Designed with biology and proteins in mind
Typically, biological samples—particularly proteins—can be a problem with pH measurements. The Biotrode reference system uses a new electrolyte called “Idrolyte,” a specialized viscous electrolyte based on a glycerol and aqueous KCl solution. Idrolyte helps to reduce the denaturing of the proteins in the sample. With its viscous nature, it also reduces any streaming potentials, allowing the Biotrode to be used with a sample that is stirring.

New trouble-free diaphragm design
Unique to the Biotrode is its new platinum diaphragm. This new system consists of plied platinum that acts as a bundle of capillaries and replaces the troublesome fritted glass diaphragm. The smooth platinum surface also greatly reduces deposits on the outside of the diaphragm.